BBS Faculty Member - Hidde Ploegh

Hidde Ploegh

Department of Pediatrics

Boston Children's Hospital
Karp Building, Room 06215
1 Blackfan Circle
Boston, MA 02115
Tel: 617-919-1613
Email: hidde.ploegh@childrens.harvard.edu



The Ploegh lab studies molecular aspects of immune recognition, using chemical, cell biological and biochemical approaches, complemented by appropriate in vivo models. We are particularly interested in the use of nanobodies (single domain antibody fragments derived from camelid heavy chain-only immunoglobulins) for non-invasive imaging by positron emission tomography. This has made it possible, for the first time, to track immune responses non-invasively, as shown for tumors in response to checkpoint blockade, next to be extended to infectious disease and autoimmunity. These same nanobodies can be modified with cytokines to re-engineer the tumor micro-environment and create more effective forms of immunotherapy. We are using Cas9/CRISPR methods to modify immunoglobulin germ line genes with sequence tags that enable site-specific modification of B cell receptors to visualize and track their behavior. These tools provide unique new models to study aspects of B cell activation that have so far defied a straight-forward analysis. Finally, the lab maintains an active interest in protein quality control, with particular emphasis on the ubiquitin-proteasome system and other post-translational modifications such as AMPylation.



Last Update: 6/19/2017



Publications

For a complete listing of publications click here.

 


 

Phenotypic lentivirus screens to identify functional single domain antibodies.
Schmidt FI, Hanke L, Morin B, Brewer R, Brusic V, Whelan SP,
Ploegh HL.
Nat Microbiol. 2016 Jun 20;1(8):16080. doi: 10.1038/nmicrobiol.2016.80.

Posttranscriptional Regulation of Glycoprotein Quality Control in the Endoplasmic Reticulum Is Controlled by the E2 Ub-Conjugating Enzyme UBC6e.
Hagiwara M, Ling J, Koenig PA,
Ploegh HL.
Mol Cell. 2016 Sep 1;63(5):753-67. doi: 10.1016/j.molcel.2016.07.014. Epub 2016 Aug 25.

The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock
70 Family Proteins, Translation Elongation Factors and Histones.
Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU,
Ploegh HL.
PLoS Genet. 2016 May 3;12(5):e1006023. doi: 10.1371/journal.pgen.1006023.

Generation of Immunity against Pathogens via Single-Domain Antibody-Antigen Constructs.
Duarte JN, Cragnolini JJ, Swee LK, Bilate AM, Bader J, Ingram JR, Rashidfarrokhi A, Fang T,
Schiepers A, Hanke L,
Ploegh HL.
J Immunol. 2016 Dec 15;197(12):4838-4847. Epub 2016 Nov 7.



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