BBS Faculty Member - Wayne Marasco

Wayne Marasco

Department of Medicine

Dana Farber Cancer Institute
450 Brookline Ave.
Boston, MA 02215
Tel: 617-632-2153
Fax: 617-632-3113
Email: wayne_marasco@dfci.harvard.edu
Visit my lab page here.



Our laboratory research focuses on the engineering of human antibodies and the use of human antibodies in discovery research and disease treatment. We are working in three disease areas: emerging infectious diseases, cancer and HIV/AIDS. We have constructed and characterized several of the largest human antibody phage display libraries ever made with tens of billions of members. Numerous human antibody engineering discoveries in our laboratory have allowed us to validate the quantity and high quality of the human antibodies in this library. Our laboratory uses all of the modern molecular techniques to isolate and characterize human monoclonal antibodies including phage, yeast and mammalian display, human CD34+ stem cell reconstructed mice, memory B-cell immortalization by EBV and TLR4 ligands, in silico modeling and co-crystallographic studies. We have all of the expertise necessary to move human Mabs into clinical trials.

Our laboratory is pursuing active research programs in human monoclonal antibody immunotherapy. In the field of infectious diseases, we are discovering and targeting highly conserved epitopes on viral glycoproteins that are recalcitrant to neutralization escape. These include influenza A and B hemagglutinin and neuraminidase, Spike protein on human coronaviruses like SARS-CoV and E-protein on flaviviruses including West Nile Virus and Denge serotypes 1-4. In the field of cancer immunotherapy, we are targeting CXCR4 and CAIX on different solid tumors such as breast cancer and renal cell carcinoma and CCR4 on cutaneous T cell lymphomas (CTCLs). We are also targeting different molecules on human T cells such as PD1-L or anti-inflammatory cytokines secreted from immune cells to augment and boost natural and vaccine induced immunity for infectious and cancer vaccines. We have also spent considerable time developing adult stem cell reconstructed humanized mouse models and several models are available to use in mechanistic and therapeutic studies (http://cfar.globalhealth.harvard.edu/icb/icb.do?keyword=k58622&pageid=icb.page416982). We also founded the National Foundation for Cancer Research Center for Therapeutic Engineering (NFCR-CTAE) to assist cancer investigators with the isolation and characterization of new anticancer human monoclonal antibodies (see http://research4.dfci.harvard.edu/nfcr-ctae/).













Last Update: 8/22/2013



Publications

For a complete listing of publications click here.

 


 

Sui J, Hwang WC, Perez S, Wei G, Aird D, Chen L, Santelli E, Stec B, Cadwell G, Ali M, Wan H, Murakami A, Yammanuru A, Han T, Cox N, Bankston LA, Donis RO, Liddington RC and Marasco WA. Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses. Nat Struct Mol Biol. 2009; 16(3):265-73. PMID: 19234466 PMCID: PMC2692245.

Han T and Marasco, WA. Structural basis of influenza virus neutralization. Ann N Y Acad Sci. The Year in Immunology. 2011 Jan;1217(1):178-90. PMID: 21251008.

Sui J, Sheehan J, Hwang WC, Bankston, LA, Burchett SK, Huang CY, Liddington R, Beigel JH, and Marasco WA. Wide prevalence of heterosubtypic broadly neutralizing human anti-Influenza A antibodies. Clinical Infectious Diseases. 2011; PMID: 21460314.

Han T, Sui J, Bennett AS, Liddington RC, Donis RO, Zhu Q and Marasco WA. Fine epitope mapping of monoclonal antibodies against hemagglutinin of a highly pathogenic H5N1 influenza virus using yeast surface display. Biochem Biophys Res Commun. 2011; Epub ahead of print. PMID: 21569761.

Herschhorn A, Marasco WA, Hizi A. Antibodies and lentiviruses that specifically recognize a T cell epitope derived from HIV-1 Nef protein and presented by HLA-C. J Immunol. 2010 Dec 15; 185(12):7623-32. PMID: 21076072.

Wrammert J, Koutsonanos D, Li GM, Edupuganti S, Sui J, Morrissey M, McCausland M, Skountzou I, Hornig M, Lipkin WI, Mehta A, Razavi B, Del Rio C, Zheng NY, Lee JH, Huang M, Ali Z, Kaur K, Andrews S, Amara R, Wang Y, Das S, Yewdell J, Subbarao K, Marasco WA, Mulligan MJ, Compans R, Ahmed R, Wilson PC. Broadly cross-reactive antibodies dominate the acute B cell response against novel 2009 H1N1 influenza virus infection. J Exp Med. 2011 Jan 17;208(1):181-93. PMID: 21220454.



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