Harvard Digestive Diseases Center

• Overview
• Administrative Core
• Imaging Core
• Epithelial Cell Biology Core
• Proteomic Core
• Gnotobiotic Animal Models Core
• Lipidomics Core

Overview:

Provides access to state of the art technologies in mass spectrometry,
microscale capillary, high performance liquid chromatography, and related
proteomics technologies required for advanced studies in cell and molecular
biology of the intestinal epithelium. A biostatistics resource is available to support
translational and basic science. This core facility is situated at Children’s Hospital
Boston in the Enders Research Building.

Director:  Hanno Steen, PhD
Research Associate: Yin Yin Lin

Contact:
Proteomics Center @ Children's Hospital Boston
Department of Pathology, Enders 1155
320 Longwood Ave
Boston, MA 02115
Phone: +1 617 919 2653
Fax: +1 617 730 0168

Services:

• Gel electrophoresis, spot picking,
• and in-gel digestion
• Protein identification by mass
• spectrometry, LC-MS/MS
• Protein modification identification and localization by LC/MS
• Determination of protein molecular
• weights by Fourier Transform Ion
• Cyclotron Resonance (FTICR) MS
• Enzymatic Digests- Gel Handling, Running SDS-Page

Equipment:

• 2D gel electrophoresis equipment
• 2D gel imaging – Typhoon/Decyder
• (GE Healthcare) Gel imager
• Nano-flow microscale capillary liquid
• chromatography (LC)
• MDLC LTQ (Thermo Scientific)
• Eksigent nanoflow HPLC system combined with LTQ-Orbitrap (Thermo Scientific)
• Surveyor HPLC combined with  LTQ-FT (Thermo Scientific)
• Nanoflow HPLC QSTAR XL (Applied Biosystems/MDS Sciex)

Fees:

Note: These are introductory fees and subject to change. All prices are in US$.

	Internal CHB Projects*	External
Protein Identification -- Gel (includes in-gel digestion, extraction, analysis and data interpretation)	US$/sample (# of samples)	US$/sample (# of samples)
Commassie stained	180 (<10) 160 (10-30) 150 (>30)	230 (<10) 190 (10-30) 175 (>30)
Silver stained**	220 (<10) 185 (10 - 30) 170 (>30)	260 (<10) 220 (10-30) 200 (>30)

 

Protein Identification from Complex Mixtures -- In Solution (includes reduction, alkylation, dialysis, 45 min instrument time and data analysis)

300

450

 

Operator/Staff Time (Data Interpretation and consulting, per hour)

100

150

 

Instrument Time (per hour, excluding operator)
LTQ	100	150
QSTAR	100	150
FT	250	350

 

Modification analysis (Coomassie-stained gels only)
First modification	300	400
Each additional modification	45	60

 

enzymatic Digests	100	150
Gel Handling (Documenting gel image, excising gel bands (up to 20 gel bands))	50	75
Running SDS-PAGE (per gel, samples provided in sample buffer by customer; includes running gel, staining, documenting and excising up to 40 bands)	200	300

 

* Children's Fund Number required
** For silver staining samples we require prior consultation in order to ensure best results.

© 2009 The President and Fellows of Harvard College.