HDDC Header Beth Israel Deaconess Medical Center Harvard Medical School Children's Hospital Boston Brigham and Woman's Hospital

CORE E: IMMUNOLOGY/MICROBIOLOGY CORE

Core Director:
Richard Blumberg, M.D.
Brigham and Women's Hospital
Department of Gastroenterology
75 Francis Street, Thorn 14
Boston, MA 02115
(617) 732-6917
Associate Scientists:
John Mekalanos, Ph.D.
Harvard Medical School
200 Longwood Avenue
D1-Rm.421
Boston, MA 02115
(617) 432-1935
.
M.R. Neutra, Ph.D.
Children's Hospital
300 Longwood Avenue
Enders 1220
Boston, MA 02115
(617) 355-6229

This Core will assist the HDDC community in:
• The isolation of murine and human intraepithelial and lamina propria lymphocytes and intestinal epithelial cells
• Performance of immunological, molecular and biochemical studies with these cell types and human intestinal tissues for specific research studies
• Immunoassays and sample collection
• Isolation and characterization of intestinal epithelial cells and mucosal mononuclear cells
• Vector repository for bacterial strains and mutants
• Growth and gene expression Microbial challenge assays.

Access to Packard Top Count-NXT 96-well plate reader, Becton-Dickinson FacsSort flow cytometer, Packard Cyclone phosphorimager, Savant SpeedVac plus and other selected instruments.