HDDC Imaging Core



	Home About Members - Full Members - Associate Members Executive Committe External Advisory Board Mini Sabbatical Award Program Core Facilities - Imaging Core - Imaging Core Fee Schedule - Epithelial Cell Biology Core - Request for cell culture - Proteomics Core - Map - Directions HDDC Seminars Symposia - Spring - Fall Pilot and Feasibility Program Contact	Imaging Core Children's Hospital: Enders 7 To arrange use of the CHILDREN'S HOSPITAL IMAGING CORE FACILITY, contact: Jessica Wagner, Research Technologist Enders Research Building, Room 706 617-919-2574 jessica.wagner@childrens.harvard.edu Technical Services offered by CH Core Technician Confocal spinning disk and deconvolution microscopy of live cells and epithelial monolayers in culture. Conventional fluorescence and light microscopy, and digital image capture of nonliving cells, tissue sections and intact mucosal tissues. Digital image processing, and preparation of figures for publication. CH Core Equipment Leica Cryostat. Reichert Ultracut E ultramicrotome for semithin and utrathin plastic sectioning; diamond knives; glass knife maker. Zeiss Axiomat photomicroscope (upright) equipped for epifluorescence, phase contrast, differential interference contrast and bright field imaging, and attached SPOT cameras and computer with image processing software. Nikon Inverted microscope with environmental chamber for live cell imaging, interfaced to a spinning disc confocal head, camera and computer with data collection, imaging and deconvolution software. Macintosh GS5 Image processing work station. Epson PRO 4000 Color Printer. Beth Israel Deaconess Medical Center (BIDMC): Dana 8 To arrange use of the BIDMC CORE FACILITIES, contact: Justine Cohen, Sr. Research Associate and Supervisor, Confocal and Light Microscopy Core Facility BIDMC, Dana 812 617-667-5373 jecohen@bidmc.harvard.edu Technical Services offered by the BIDMC Core Electron microscopy (EM). Ultrathin sectioning and EM of tissues, isolated cells, epithelial monolayers, and subcellular fractions; direct ultrastructural analysis, localization of tracers and antigens, and quantitative analysis. Tissue preparation. Embedding of tissues in plastic resin for EM, semithin or ultrathin conventional and cryo-sectioning and staining for light, fluorescence or electron microscopy. Paraffin histology. Processing, embedding, and sectioning of fixed tissues for H&E paraffin histology. Special stains are also available, like PAS. Confocal laser scanning microscopy: Confocal imaging of fixed cells or of tissue sections. Conventional fluorescence and light microscopy. Light (color or black/white) and widefield imaging of tissue sections with computer assisted image capture. Photography, digital image processing, 3D image reconstruction and preparation of figures for publication. BIDMC Core Equipment Leica Cryostat. Reichert Ultracut E ultramicrotomes for semithin and ultrathin plastic sectioning and a Reichert Ultracut S cryo-ultramicrotome for cryosectioning; diamond knives; glass knife maker. JEOL 1200 EX Electron microscope. Conventional light/fluorescence photomicroscopes (Nikon E800 with a SPOT camera). ZEISS LSM510-META upright laser confocal microscope for imaging frozen sections or fixed cultured cells. ZEISS LSM510-META Inverted laser confocal microscope and incubator for live cell imaging (projected for use in Spring of 2007). Image processing computer work stations and Fuji Pictrography printer. INSTRUCTIONS FOR ACCESS Contact the Sr. Associate, either Jessica Wagner at CH or Justine Cohen at BIDMC. Identify yourself as an HDDC member, Associate Member, or PF recipient. This step is required if you wish to receive the free service/equipment usage offer during the introductory period of July 1-December 1 of 2006. Discuss what you or your trainee would like to do in the core and make an appointment for service or for training. If you have not yet used either core, ask for a tour of the facility. At the time you begin use of the core, be sure you or your trainee gives the Sr. Associate the location of your lab, the title of the grant that supports your work, and the relevant NIH grant number. This information is essential for renewal of the HDDC grant. Obtain the appropriate access card to utilize core equipment or service before your appointment. YOU MUST HAVE A SENSOR CARD to access the BIDMC core and you must bring along an HMS or affiliated hospital ID card to use the CH core. Obtain training on all core equipment prior to independent usage. All training for HDDC members is FREE. Please note that training is not considered part of your $500 bonus for core use in the introductory period. At the training session, you will be informed about how to access the HDDC equipment calendars so that you can sign-up for independent use. If it is service you require, make an appointment to drop-off your samples or to discuss any special orientation you require. Please make an appointment if you wish to have special help, as the technicians are very busy with scheduled work during the day and may not be available to help if you just “drop-in”. If payment is required, please obtain a PO# or provide a valid and active account #. We hope you have a fruitful experience using the cores. If ANY problems arise that are not immediately fixed by the Sr. Associates, contact Dr. Neutra or Dr. Hagen immediately. USER FEES The costs of running the HDDC Imaging Core include maintenance of equipment, supplies, and the salary of several expert technicians. The HDDC NIH Center Grant and the resources of Core Scientists provide partial coverage of these costs. To cover the shortfall, we must ask Core users to contribute user fees. Previously, regular users of the Core paid a yearly subscription fee for preferred access and unlimited use of certain Core equipment, while others were charged by the hour for use of the equipment and technicians. This scheme was rather complex and often did not reflect actual use. As of July 1, 2006, we are offering a new introductory plan to encourage Core use, and a new cost-sharing plan that is more straightforward and fair. At the end of the first grant period (Dec. 1, 2006) we will evaluate this plan and make adjustments as needed. Here are the key points: HDDC Members and Associate Members (and their trainees) and pilot/feasibility grant recipients have preferred access to Core facilities. Consultation, protocols and basic training in the use of equipment (confocal and conventional photomicroscopes, live cell imaging equipment, electron microscope, cryostat, etc.) are provided to HDDC affiliates at no cost. See details below. Each Member, Associate Member, and P/F grantee is entitled to free use of Core equipment and services, equivalent to $500 worth of billable use in the introductory period from July 1 to Dec. 1, 2006. This offer applies to all, whether or not the investigator is currently using the Core. Equipment use or services over and above the $500 limit will incur charges according to a fixed schedule of user fees. These fees have been calculated to cover Core expenses that are not covered by the HDDC grant. A detailed schedule of user fees follows. Note that the user fees charged to HDDC members are lower than those charged to non-members. The fee schedule for HDDC members and non-members in convenient table format is also attached to this email. Services offered at no charge: 1. Expert consultation, advice and protocols provided by Core scientists and staff. 2. Basic training in preparation for independent or assisted use of Core equipment, including: Electron microscope Conventional upright photomicroscopes/cameras/computers for fluorescence, phase contrast, differential interference contrast, and deconvolution microscopy. Laser scanning confocal microscopes. Inverted fluo rescence microscope with spinning disc for live cell imaging. Inverted fluo rescence photomicroscopes/cameras/computers with deconvolution capability. Cryostats for frozen sectioning. An older IEC microtome, rather than the Leica, is available for independent usage at the BIDMC core facility. Ultramicrotomes for semithin and ultrathin sectioning. Please note that training is for equipment only and does not include teaching the technique of microscopy or microtomy to users. 3. Advanced training courses may be offered for individuals or groups as needed; subjects may include: Advanced confocal, live cell and deconvolution microscopy. EM: Preparation of tissues and cells, EM imaging and image interpretation. Immunocytochemistry: Optimizing fixation and immunostaining protocols for antigen preservation, primary and secondary reagents, visualization of multiple antigens. Image analysis: computer based production, capture and analysis of high resolution images, CONTACT INFORMATION FOR THE HDDC IMAGING CORE Marian R. Neutra, Director Enders Research Building, Room 720 617-919-2575 617-730-0498 (fax) marian.neutra@childrens.harvard.edu Susan J. Hagen, Scientist BIDMC, Dana 805 617-667-5308 (office) 617-667-1807 (Assistant, Andrew French) 617-975-5562 (fax) shagen@caregroup.harvard.edu Tomas Kirchhausen, Scientist Center for Blood Research Warren Alpert Building, Room 134 617-278-3140 617-278-3125 (Assistant, Caitlin Lynch) 617-278-3115 (fax) kirchhausen@crystal.harvard.edu Josh Rosenfeld, Administrator Enders Research Building, Room 720 617-919-2546 617-730-0498 (fax) joshua.rosenfeld@childrens.harvard.edu Jessica Wagner, Senior Technician Enders Research Building, Room 706 617-919-2574 jessica.wagner@childrens.harvard.edu Justine Cohen, Sr. Research Associate and Supervisor, Confocal and Light Microscopy Core Facility BIDMC, Dana 812 617-667-5373 jecohen@bidmc.harvard.edu Suzanne White, Sr. Histotechnologist and Supervisor, Histology Core Facility BIDMC, Dana 806 617-667-4117 swhite1@bidmc.harvard.edu Wendy Litzkow, Histotechnologist BIDMC, Dana 812 617-667-1828 wlitzkow@bidmc.harvard.edu


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