Virology Faculty Member - Priscilla Yang

Priscilla Yang

Harvard Medical School
MBIB, NRB, Room 930
77 Avenue Louis Pasteur
Boston, MA 02115
Tel: 617-432-5415
Fax: 617-432-5418
Email: priscilla_yang@hms.harvard.edu
Visit my lab page here.



My group’s focus has been on the study of mammalian viruses that are significant causes of morbidity and mortality in humans, in particular hepatitis B virus (HBV), hepatitis C virus (HCV), and dengue virus. We are especially interested in using chemical tools to elucidate molecular mechanisms underlying the replication of viral pathogens and to validate new antiviral targets and strategies. Current themes in my laboratory include the use of small molecules and RNAi as complementary tools to interrogate the role of specific host factors in viral replication; the use of mass spectrometry-based methods to investigate the structure and function of specific lipids in viral replication; the use of activity-based chemoproteomic profiling to characterize virus-induced changes in the host NTPome; exploitation of the host reactive “cysteineome” to develop irreversible, host-targeted antiviral compounds; and the investigation of polypharmacology as a strategy to develop more effective antivirals with higher barriers to resistance.




































Last Update: 4/1/2014



Publications

de Wispelaere, M., LaCroix, A., and Yang, P.L. (2013) The Small Molecules AZD0530 and Dasatinib Inhibit Dengue Virus RNA Replication via Fyn kinase. Journal of Virology, published ahead of print 24 April 2013, doi: 10.1128/JVI.00632-13. PMCID: PMC3700292.

Vetter, M.L., Rodgers, M.A., Patricelli, M.P., and Yang, P.L. (2012) Chemoproteomic Profiling Identifies Changes in DNA-PK as Markers of Early Dengue Virus Infection. ACS Chemical Biology, 7(12):2019-2026. NIHMSID # 410237, PMCID: PMC3528803. Chosen for the cover and author highlight

Zhang, Z., Kwiatkowski, N., Zeng, H., Lim, S.M., Gray, N.S., Zhang, W., and Yang, P.L. (2012) Leveraging kinase inhibitors to develop small molecule tools for imaging kinases by fluorescence microscopy. Mol. BioSyst., 8(10):2523-2526. PMCID: PMC3616611. Highlighted by the journal as a HOT Article

de Wispelaere, M. and Yang, P.L. (2012) Mutagenesis of the domain I - domain III linker in the Dengue Virus envelope protein impairs viral particle assembly. Journal of Virology, 86(13):7072-7083. PMCID: PMC3416339.

Rodgers, M.A., Villareal, V.A., Schaefer, E.A., Peng, L.F., Corey, K.E., Chung, R.T., and Yang, P.L. (2012) Lipid metabolite profiling identifies desmosterol metabolism as a new antiviral target for hepatitis C virus. Journal of the American Chemical Society, 134(16):6896-6899. PMCID: PMC3375380.

Schmidt, A., Lee, K., Yang, P.L., and Harrison, S.C. (2012) Small molecules of dengue virus entry. PLoS Pathogens 8(4): e1002627. PMCID: PMC3320583.

Schmidt, A., Yang, P.L., and Harrison, S.C. (2010) Peptide inhibitors of flavivirus entry derived from the E-protein stem. Journal of Virology, 84(24):12549-54. PMCID: PMC3004314.

Schmidt, A., Yang, P.L., and Harrison, S.C. (2010) Peptide inhibitors of dengue-virus entry target a late-stage fusion intermediate. PLOS Pathogens 6(4):e1000851.doi:10.1371/journal.ppat.1000851. PMCID: PMC2851732

Sessions, O.M., Barrows, N.J., Souza-Neto, J.A., Robinson, T.J., Hershey, C.L., Rodgers, M.A., Ramirez, J.L., Dimopoulos, G., Yang, P.L., Pearson, J.L., and Garcia-Blanco, M.A. (2009). Discovery of dengue virus host factors in insects and humans. Nature, 458(7241), 1047-1050. PMCID: PMC3462662.



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