Virology Faculty Member - Peter Howley

Peter Howley

Shattuck Professor of Pathological Anatomy
Department of Microbiology and Immunobiology

Harvard Medical School
New Research Building, Room 950a
77 Avenue Louis Pasteur
Boston, MA 02115
Tel: 617-432-2889
Fax: 617-432-2882
Email: peter_howley@hms.harvard.edu
Lab Members: 5 postdoctoral fellows, 2 graduate students



Research in the Howley laboratory is focused on the molecular biology of the papillomaviruses and the role of these viruses in carcinogenesis. Several specific types of the human papillomaviruses (HPVs) are associated with human cervical cancer. The "high risk" HPVs encode two oncoproteins, E6 and E7, which target the tumor suppressor gene proteins p53 and pRB, respectively. We have previously shown that E6 promotes the ubiquitylation and degradation of p53, and are now interested in the general question of how proteins are recognized within cell by the ubiquitin proteolytic machinery. The E6 promoted ubiquitylation of p53 is mediated by the cellular protein E6AP (also known as UBE3A) that complexes with E6 to directly target p53 for ubiquitylation. We are interested in identifying the genes that regulate E6 mediated proteolysis of p53 and in screening for small molecule inhibitors of this process.

One major area of research in the laboratory has focused on the E2 regulatory proteins encoded by the papillomaviruses. The full length and spliced versions of E2 protein regulate expression of the viral E6 and E7 oncoproteins, have auxiliary roles in viral DNA replication and play a role in viral genome maintenance for establishing persistent infections. We identified the bromodomain protein Brd4 as a key cellular binding partner of E2 that mediates a number of these important functions. Projects in the laboratory involving the viral E2 protein employ proteomic and genome wide siRNA approaches to identify cellular proteins and pathways involved in HPV virus host cell interactions.

Our interests extend to other aspects of papillomavirus-host cell interactions, including how these viruses evade innate and adaptive host cell immune recognition. Another area of study in the laboratory involves the characterization of the human papillomavirus protein network using mass spectrometry to generate a high quality network interaction map encompassing proteins encoded by the oncogenic as well as non-oncogenic HPV types.

Rotation projects are available in each of these areas.



Last Update: 10/22/2013



Publications

Rahman, S., Sowa, M.E., Ottinger, M., Smith, J.A., Shi, Y., Harper, J.W., and Howley, P.M. The Brd4 Extraterminal (ET) Domain confers transcription activation independent of pTEFb by recruiting multiple proteins including NSD3. Mol. and Cell. Biol. 31:2641-2652, 2011.

White, E.A., Sowa, M.E., Tan, M.J.A., Jeudy, S., Hayes, S.D., Shantha, S., Münger, K., Harper, J.W., and Howley, P.M. Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses. Proc. Nat. Acad. Sci. USA 109:E260-E267, 2012.

Tan, M.J.A., White, E.A., Sowa, M.E., Harper, J.W., Aster, J.C. and Howley, P.M. Cutaneous beta-human papillomavirus E6 proteins bind mastermind-like co-activators and repress Notch signaling. Proc. Nat. Acad. Sci. USA 109:E1473-E1480, 2012.

Martinez-Noël, G., Galligan, J.T., Sowa, M.E., Arndt, V., Overton, T.M., Harper, J.W., and Howley, P.M. Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes. Mol. Cell. Biol., 32:3095-3106, 2012.

White, E.A., Kramer, R.E., Tan, M.J.A., Hayes, S.D., Harper, J.W., and Howley, P.M. Comprehensive Analysis of Host Cellular Interactions with Human Papillomavirus E6 Proteins Identifies New E6 Binding Partners and Reflects Viral Diversity. J. Virol. 86:13174-13186, 2012.



© 2013 by the President and Fellows of Harvard College