Dana Gabuzda, M.D.
Associate Head, Virology Program
Professor of Neurology, (Microbiology and Molecular Genetics)
44 Binney Street
Boston, MA 2115
The major research interest of the lab is to understand molecular mechanisms of HIV replication and pathogenesis, and their implications for understanding pathogen-host interactions, immune responses, and disease outcomes. Using biological, genetic, biochemical, and systems biology approaches, we study: 1) HIV replication and pathogenesis in the immune system, gut, and central nervous system; 2) mechanisms and pathways underlying differences in host responses to viral infection and antiretroviral therapy; 3) viral diversity and tropism for specific cell types and tissues; 4) functions of the HIV Env, Vif, and Nef proteins; 5) high-throughput screening assays to identify small molecules that inhibit Vif interaction with its host cell cofactor APOBEC3G.
Dunfee RL, Thomas ER, Gorry PR, Taylor J, Kunstman K. Bell JM, Wolinsky SM, Gabuzda D. The HIV Env variant N283 enhances macrophage tropism and is associated with brain infection and dementia. Proc. Natl. Acad. Sci. USA 2006.
Thomas ER, Dunfee RL, Stanton J, Bogdan D, Taylor J, Kunstman K, Bell JE, Wolinsky SM, Gabuzda D. Macrophage entry mediated by HIV Envs from brain and lymphoid tissues is determined by the capacity to use low CD4 levels and overall efficiency of fusion. Virology 2007.
Mehle A, Wilson H, Zhang C, Brazier AJ, McPike M, Pery E Gabuzda D. Identification of an APOBEC3G binding site in HIV Vif and inhibitors of Vif-APOBEC3G binding. J. Virol. 2007.
Mefford ME, Gorry PR, Kunstman K, Wolinsky SM, Gabuzda, D. Bioinformatic prediction programs underestimate the frequency of CXCR4 usage by R5X4 HIV in brain and other tissues. AIDS Res. Human Retroviruses 2008.
Ancuta P, Kamat A, Kunstman KJ E, Autissier P, Wurcel A, Zaman T, Stone D, Mefford M, Morgello S, Singer EJ, Wolinsky SM, Gabuzda D. Microbial translocation is associated with increased monocyte activation and dementia in AIDS patients. PLoS ONE 2008.
Pery E, Rajendran KS, Brazier AJ, Gabuzda D. Regulation of APOBEC3 proteins by a novel YxxL motif in HIV and SIVagm Vif. J Virol 2009.
Dunfee RL, Thomas ER, Gabuzda D. Enhanced macrophage tropism of HIV in brain and lymphoid tissues is associated with sensitivity to the broadly neutralizing CD4 binding site antibody b12. Retrovirology 2009
Ancuta P, Liu KY, Misra V, Wacleche VS, Gosselin A, Zhou X, Gabuzda D. Transcriptional profiling reveals developmental relationship and distinct biological functions of CD16+ and CD16- monocyte subsets. BMC Genomics 2009.
Olivieri KC, Agopian KA, Mukerji J, Gabuzda D. Evidence for adaptive evolution at the divergence between lymphoid and brain HIV nef genes. AIDS Res Human Retroviruses 2010