Immunology Faculty Member - Hongbo Luo, PhD

Hongbo Luo, PhD

Department of Lab Medicine, Boston Children's Hospital
811 Enders Building
320 Longwood Ave.
Boston, MA 02115
Tel: 617-910-2303
Fax: 617-730-0885
Email: hongbo.luo@childrens.harvard.edu
Visit my lab page here.



One of the major players in innate immune system is neutrophil which is the most abundant cell type among circulating white blood cells and constitutes the first line of host defense against invading bacteria and other pathogens. The long-term objective of our research is to elucidate the molecular mechanisms and cellular signaling pathways that control various neutrophil functions. The ultimate goal is to control the number and function of neutrophils during infection and inflammation. Currently, we are particularly interested in signaling pathways mediated by inositol phospholipid PtdIns(3,4,5)P3, inositol phosphates (e.g. InsP4), and reactive oxygen species (ROS). We utilize a wide variety of approaches ranging from basic molecular and cell biology methods to high throughput chemical genetic screening and animal inflammation models to dissect these pathways.

Ongoing studies:
1) Signal pathways mediating neutrophil directional movement.
2) Molecular mechanism of neutrophil spontaneous cell death.
3) Neutrophil homeostasis under normal and inflammatory conditions.
4) Regulation of neutrophil function in bacterial pneumonia.
5) Improving neutrophil performance in granulocyte transfusion.
6) Regulation of hematopoiesis by neutrophils.



















Last Update: 6/18/2018



Publications

Liang OD, Lu J, Nombela-Arrieta C, Zhong J, Zhao L, Pivarnik G, Mondal S, Chai L, Silberstein LE, and Luo HR. Deficiency of Lipid Phosphatase SHIP Enables Long Term Reconstitution of Hematopoietic Inductive Bone Marrow Microenvironment. Developmental Cell. 2013;25(4):333-349.

Xu Y, Li H, Bajrami B, Kwak H, Cao S. Liu P, Zhou J, Zhou Y, Zhu H, Ye K, and Luo HR. Cigarette smoke (CS) and nicotine delay neutrophil spontaneous death via suppressing production of diphosphoinositol pentakisphosphate. Proc Natl Acad Sci U S A. 2013;110(19):7726-7731.

Jo H, Loison F, and Luo HR. Microtubule dynamics regulates Akt signaling via dynactin p150. Cell Signal. 2014;26(8):1707-1716. PMID: 24726838

Loison F, Zhu H, Karatepe K, Kasorn A, Liu P, Ye K, Zhou J, Cao S, Gong H, Jenne DE, Remold-O'Donnell E, Xu Y, and HR Luo. Proteinase 3-dependent caspase-3 cleavage modulates neutrophil death and inflammation. J Clin Invest. 2014;124(10):4445-4458. PMCID: PMC4191030.

Luo HR and Mondal S. Molecular control of PtdIns(3,4,5)P3 signaling in neutrophils. EMBO Rep. 2015;16(2):149-163.

Kwak HJ, Liu P, Bajrami B, Xu Y, Park SY, Nombela-Arrieta C, Mondal S, Sun Y, Zhu H, Chai L, Silberstein LE, Cheng T, and Luo HR. Myeloid cell-derived reactive oxygen species externally regulate the proliferation of myeloid progenitors in emergency granulopoiesis. Immunity. 2015;42(1):159-171.

Besnik Bajrami, Haiyan Zhu, Hyun-Jeong Kwak, Subhanjan Mondal, Qingming Hou, Guangfeng Geng, Kutay Karatepe, Yu C. Zhang, Cesar Nombela-Arrieta, Shin-Young Park, Fabien Loison, Jiro Sakai, Yuanfu Xu, Leslie E. Silberstein, and Hongbo R. Luo. G-CSF maintains controlled neutrophil mobilization during acute inflammation by negatively regulating CXCR2 signaling. J Exp Med. 2016 Sep 19;213(10):1999-2018.

Teng, Y., Luo, H.R., and Kambara, H. (2017). Heterogeneity of neutrophil spontaneous death. Am J Hematol. 92(8):E156-E159.

Zhu, H., H. J. Kwak, P. Liu, B. Bajrami, Y. Xu, S. Y. Park, C. Nombela-Arrieta, S. Mondal, H. Kambara, H. Yu, L. Chai, L. E. Silberstein, T. Cheng and H. R. Luo. (2017). Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis. J Immunol 2016 198, 2854-2864.

Xue Zhang, Peng Liu, Christie Zhang, Direkrit Chiewchengchol, Fan Zhao, Hongbo Yu, Jingyu Li, Hiroto Kambara, Kate Y. Luo, Arvind Venkataraman, Ziling Zhou, Weidong Zhou, Haiyan Zhu, Li Zhao, Jiro Sakai, Yuanyuan Chen, Ye-Shih Ho, Besnik Bajrami, Bing Xu, Leslie E. Silberstein, Tao Cheng, Yuanfu Xu, Yuehai Ke and Hongbo R. Luo. Positive regulation of interleukin-1 beta bioactivity by physiological ROS-mediated Cysteine S-glutathionylation. Cell reports, 2017. 20, 224-235.

Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S, Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR. Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Cell reports. 2018;22(11):2924-2936.

Hou Q, Liu F, Chakraborty A, Jia Y, Prasad A, Yu H, Zhao L, Ye K, Snyder SH, Xu Y, Luo HR. Inhibition of IP6K1 suppresses neutrophil-mediated pulmonary damage in bacterial pneumonia. Science Translational Medicine. 2018;10(435).



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