We seek to understand how coat proteins initiate intracellular transport, and have found that the GTPase-activating proteins (GAPs) for the ADP-Ribosylation Factor (ARF) small GTPases act not only conventionally as upstream regulators of ARFs but also as novel downstream effectors, by being key components of coat complexes. This insight has led us to identify additional factors critical for the formation of transport vesicles. We are also applying these insights to elucidating intracellular transport mechanisms that underlie cell migration, glucose homeostasis, and viral replication.
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References:
- Lee, SY, Yang, JS, Hong, W, Premont, RT, and Hsu, VW. ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation. Journal of Cell Biology 2005; 168: 281-90.
- Li, J., Ballif, BA, Powelka, AM, Dai, J., Gygi, SP, Hsu, VW. Phosphorylation of ACAP1 by Akt regulates the stimulation-dependent recycling of integrin beta1 to control cell migration. Developmental Cell 2005; 9: 663-73.
- Yang, JS, Zhang, L, Lee, SY, Gad, H, Luini, A, and Hsu, VW. Key components of the fission machinery are interchangeable. Nature Cell Biology 2006; 8: 1376-82.
- Li, J, Peters, PJ, Bai, M., Dai, J., Bos, E., Kirchhausen, T., Kandror, KV, Hsu, VW. An ACAP1-containing clathrin coat complex for endocytic recycling. Journal of Cell Biology 2007; 178: 453-464.
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