BBS Faculty Member - Yi Zhang

Yi Zhang

Department of Genetics

Boston Children's Hospital
Warren Alpert Building, Room 149G
200 Longwood Ave.
Boston, MA 02115
Tel: 617-713-8666
Fax: 617-713-8665
Visit my lab page here.

The Zhang lab is interested in how epigenetic modification-mediated dynamic changes in chromatin structure affects gene expression, stem cell reprogramming, germ cell development, beta-cell generation, and drug addiction. In the past decade, the lab has worked on a number of projects that span many aspects of epigenetic modifications that include: 1) ATP-dependent nucleosome-remodeling and deacetylase complex NuRD; 2) various histone methyltransferases, including PRC2 (EZH2) and DOT1L; 3) various histone demethylases such as JmjC family demethylases; 4) histone H2A ubiquitin E3 ligase PRC1; and 5) the Tet family of 5-methylsytosine dioxygenases. Build on the past 15 years of success, the lab has developed and adapted new technologies to meet the new challenges.

The lab is using cutting-edge technologies, such as live cell imaging, protein depletion in developing oocytes, single-cell RNA-seq and RRBS, to understand the role of dynamic changes
DNA methylation and histone modifications in regulating gene expression during early embryogenesis, germ cell development, stem cell reprogramming (iPS and SCNT), cancer drug resistance, beta-cell regeneration and neurogenesis. The available rotation projects include:

1) epigenetic mechanism of cancer drug resistance;
2) mechanism of pluripotency and totipotency conversion;
3) mechanism of iPSC generation and SCNT-mediated reprogramming;
4) CpG island chromatin formation;
5) Role of Tet and 5mC oxidation products in differentiation and brain function.

Last Update: 6/24/2014


For a complete listing of publications click here.



Inoue, A., and Zhang, Y. (2014). Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes. Nature SMB (Jun 8, Epub ahead of print).

Wang, Y., and
Zhang, Y. (2014). Regulation of TET protein stability by calpains. Cell Reports 6, 278-84.

Wu, H., and
Zhang, Y. (2014). Reversing DNA methylation: mechanisms, genomics, and biological functions. Cell 156, 45-68.

Yamaguchi, S., Shen, L., Liu, Y., Sendler, D., &
Zhang, Y. (2013). Role of Tet1 in erasure of genomic imprinting. Nature 504, 460-64.

Kohli, R.M., and
Zhang, Y. (2013). TET enzymes, TDG and the dynamics of DNA demethylation. Nature 502, 472-9.

Ran, F.A., Hsu, P.D., Lin, C-Y., Gootenberg, J.S., Konermann, S., Trevino, A., Scott, D.A., Inoue, A., Matoba, S.,
Zhang, Y., and Zhang, F. (2013). Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154, 1380-1389.

Shen, L., Wu, H., Diep, D., D’Alessio, A. C., Fung, A., Zhang, K., and
Zhang, Y. (2013). Genome-wide analysis reveals TET and TDG-mediated 5-methylcytosine oxidation dynamics. Cell 153, 692-706.

He, J., Shen, L., Wan, M., Taranova, O., Wu, H., &
Zhang, Y. (2013). Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of early lineage genes. Nature Cell Biol 15, 373-384.

Yamaguchi, S., Hong, K., Liu, R., Shen, L., Inoue, A., Diep, D., Zhang, K., and
Zhang, Y. (2012). Tet1 controls meiosis by regulating meiotic gene expression. Nature 492, 443-447.

Liang, G., He, J., and
Zhang, Y. (2012). Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming. Nature Cell Biol. 14, 457-466.

© 2013 by the President and Fellows of Harvard College