BBS Faculty Member - Elizabeth Henske

Elizabeth Henske

Department of Medicine

Brigham and Women's Hospital
Harvard Medical School
One Blackfan Circle, Karp Bldg. 6th Floor
Boston, MA 02115
Tel: 617-355-9049
Fax: 617-355-9016
Email: ehenske@partners.org
Visit my lab page here.



My laboratory is focused on two tumor suppressor gene diseases associated with renal tumorigenesis and hamartomas: tuberous sclerosis complex (TSC) and Birt-Hogg-Dube (BHD) syndrome. I have a particular interest in lymphangioleiomyomatosis (LAM), which is the pulmonary manifestation of TSC. My group is perhaps best known for discovering that LAM is caused by somatic TSC2 mutations, and that LAM cells metastasize despite the fact that they are histologically benign, leading to the now widely accepted "benign metastasis" model. Remarkably, LAM affects almost exclusively young women. The mechanisms underlying this female predominance are a major focus of my laboratory.

In addition to studying human tumor specimens from patients with these diseases, we use cultured cells, mice, and
Schizosaccharomyces pombe as models. Our work has led to basic discoveries related to regulation of the mTOR signaling pathway by the cell cycle and by estrogen and regulation of amino acid homeostasis by the TSC and BHD homologs in yeast. We were the first to demonstrate that the protein products of the TSC1 and TSC2 genes (hamartin and tuberin, respectively) physically interact and reported the the first post-translational modification of the TSC1 protein (CDK1 phosphorylation). We generated the first yeast model of BHD and the first heterozygous mouse model of BHD. BHD and TSC have distinct clinical similarities, including facial hamartomas, chromophobe renal cell carcinomas, and spontaneous pneumothorax (lung collapse). Given these similarities, our work in S. Pombe has yielded surprising results: the TSC and BHD protein homologs have opposing roles in the regulation of amino acid homeostasis, including regulation of amino acid permeases and intracellular amino acid levels. We remain intensely interested in the function of the BHD protein, using cell culture and mouse models in addition to our yeast model. My research program is by its nature interdisciplinary, bringing together different areas of medicine and biology. The mechanisms we study have relevance to cell biology, genetics, pathology, and developmental biology, and the models we study include humans, mice, and yeast.



Last Update: 8/22/2013



Publications

For a complete listing of publications click here.

 


 

van Slegtenhorst M, Khabibulllin D, Hartman TR, Nicolas E, Kruger WD, Henske EP. The Birt-Hogg-Dube and Tuberous Sclerosis Complex homologs have opposing roles in amino acid homeostasis in Schizosaccharomyces pombe. J Biol Chem 282: 24583-24590, 2007.

Yu J, Robb VA, Morrison TA, Ariazi EA, Karbowniczek M, Astrinidis A, Wang C, Hernandez-Cuebas L, Seeholzer LF, Nicolas E, Hensley H, Jordan VC, Walker CL,
Henske EP. Estrogen promotes the survival and pulmonary metastasis of tuberin-null cells, Proc Natl Acad Sci USA. 106: 2635-2640, 2009 (Track II). PMC2637277

Karbowniczek M, Zitserman, D, Khabibullin D, Hartman, T, Yu J, Morrison T, Nicolas, E, Squillace R, Roegiers F and
Henske EP. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. The Journal of Clinical Investigation 120:93-102, 2010. PMID: 20038815

Parkhitko A, Myachina F, Morrison TA, Hindi KM, Auricchio N, Karbowniczek M, Wu JJ, Finkel T, Kwiatkowski DJ, Yu JJ, and
Henske EP. Tumorigenesis in tuberous sclerosis complex is autophagy and p62/sequestosome 1 (SQSTM1)-dependent. PNAS, published online July 2011.



© 2013 by the President and Fellows of Harvard College