BBS Faculty Member - David Clapham

David Clapham

Department of Cardiology, HHMI, BCH
Department of Neurobiology, HMS

Boston Children's Hospital
Enders Bldg., Rm. 1309
320 Longwood Ave.
Boston, MA 02115
Tel: 617-919-2680
Fax: 617-731-0787
Visit my lab page here and here.

We study signal transduction, primarily the control of ion channels in heart and brain. We study the molecules that control the entry and release of intracellular calcium, and the mechanisms of calcium control of cell function.

Last Update: 6/2/2014


For a complete listing of publications click here.



Zhang X, Ren W, DeCaen P, Yan C, Tao X, Tang L, Wang J, Hasegawa K, Kumasaka T, He J, Wang J, Clapham DE, and N. Y. 2012. Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel. Nature 486: 130-135.

Ye L, Kleiner S, Wu J, Sah R, Gupta RK, Banks AS, Cohen P, Khandekar MJ, Bostrom P, Mepani RJ, Laznik D, Kamenecka TM, Song X, Liedtke W, Mootha VK, Puigserver P, Griffin PR, Clapham DE, and Spiegelman BM. 2012. TRPV4 Is a Regulator of Adipose Oxidative Metabolism, Inflammation, and Energy Homeostasis.
Cell 151: 96-110.

Wu LJ, Wu G, Sharif MR, Baker A, Jia Y, Fahey FH, Luo HR, Feener EP, and Clapham DE. 2012. The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke.
Nature Neuroscience 15: 565-573.

Wang X, Zhang X, Dong X-p, Samie M, Li X, Cheng X, Goschka A, Shen D, Zhou Y, Harlow J, Zhu MX, Clapham DE, Ren D, and Xu H. 2012. TPC Proteins Are Phosphoinositide- Activated Sodium-Selective Ion Channels in Endosomes and Lysosomes.
Cell 151: 372-383.

Stotz SC and Clapham DE. 2012. Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screen.
PLOS One 7: e46865.

Kaczmarek JS, Riccio A, and Clapham DE. 2012. Calpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A-induced neuronal growth cone collapse.
Proc Natl Acad Sci U S A 109: 7888-7892.

Doerner JF, Febvay S, and Clapham DE. 2012. Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscL.
Nature communications 3: 990.

Desai BN, Krapivinsky G, Navarro B, Krapivinsky L, Carter BC, Febvay S, Delling D, Penumaka A, Ramsey IS, Manasian Y, and Clapham DE. 2012. Cleavage of TRPM7 releases the kinase domain from the ion channel and regulates its participation in Fas-induced apoptosis.
Dev Cell 22: 1149-1162.

Zimmermann K, Lennerz JK, Hein A, Link AS, Kaczmarek JS, Delling M, Uysal S, Pfeifer JD, Riccio A, and Clapham DE. 2011. Transient receptor potential cation channel, subfamily C, member 5 (TRPC5) is a cold-transducer in the peripheral nervous system.
Proc Natl Acad Sci U S A 108: 18114–18115.

Xue T, Do MTH, Riccio A, Jiang Z, Hsieh J, Wang HC, Merbs SL, Welsbie DS, Yoshioka T, Weissgerber P, Stolz S, Flockerzi V, Freichel M, Simon MI, Clapham DE, and Yau KW. 2011. Melanopsin signalling in mammalian iris and retina.
Nature 479: 67-73.

Navarro B, Miki K, and Clapham DE. 2011. ATP-activated P2X2 current in mouse spermatozoa.
Proc Natl Acad Sci U S A 108: 14342-14347.

Krapivinsky G, Krapivinsky L, Stotz SC, Manasian Y, and Clapham DE. 2011. POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.
Proc Natl Acad Sci U S A 108: 19234–19239.

Jin J, Wu L-J, Jun J, Cheng X, Xu H, Andrews NC, and Clapham DE. 2011. The channel kinase, TRPM7, is required for early embryonic development
Proc Natl Acad Sci U S A (PNAS Plus) 109: 1365-1366; E1225-E1233.

Clapham DE and Miller C. 2011. A thermodynamic framework for understanding temperature sensing by transient receptor potential (TRP) channels.
Proc Natl Acad Sci U S A 108: 19492-19497.

Chung JJ, Navarro B, Krapivinsky G, Krapivinsky L, and Clapham DE. 2011. A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa.
Nat Commun 2: 153-165.

Cai X and Clapham DE. 2011. Ancestral Ca2+ Signaling Machinery in Early Animal and Fungal Evolution.
Molecular biology and evolution 29: 91-100.

Ramsey IS, Mokrab Y, Carvacho I, Sands ZA, Sansom MS, and Clapham DE. 2010. An aqueous H+ permeation pathway in the voltage-gated proton channel Hv1.
Nat Struct Mol Biol 17 869-875.

Febvay S, Marini DM, Belcher AM, and Clapham DE. 2010. Targeted cytosolic delivery of cell-impermeable compounds by nanoparticle-mediated, light-triggered endosome disruption.
Nano Lett 10: 2211-2219.

Cheng X, Jin J, Hu L, Shen D, Dong XP, Samie MA, Knoff J, Eisinger B, Liu ML, Huang SM, Caterina MJ, Dempsey P, Michael LE, Dlugosz AA, Andrews NC, Clapham DE, and Xu H. 2010. TRP channel regulates EGFR signaling in hair morphogenesis and skin barrier formation.
Cell 141: 331-343.

Zimmermann K, Hein A, Hager U, Kaczmarek JS, Turnquist BP, Clapham DE, and Reeh PW. 2009. Phenotyping sensory nerve endings in vitro in the mouse.
Nat Protoc 4: 174-196.

Zhou H and Clapham DE. 2009. Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development.
Proc Natl Acad Sci USA 106: 15750-15755. PMCID: PMC2732712.

Riccio A, Li Y, Moon J, Kim KS, Smith KS, Rudolph U, Gapon S, Yao GL, Tsvetkov E, Rodig SJ, Van't Veer A, Meloni EG, Carlezon WA, Jr., Bolshakov VY, and Clapham DE. 2009. Essential role for TRPC5 in amygdala function and fear-related behavior.
Cell 137: 761-772. PMCID: PMC2719954.

Ramsey IS, Ruchti E, Kaczmarek JS, and Clapham DE. 2009. Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst.
Proc Natl Acad Sci USA 106: 7642-7647. PMCID: PMC2669790.

Oancea E, Vriens J, Brauchi S, Jun J, Splawski I, and Clapham DE. 2009. TRPM1 forms ion channels associated with melanin content in melanocytes.
Sci Signal 2: ra21.

Jiang D, Zhao L, and Clapham DE. 2009. Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca²?/H? antiporter.
Science 326: 144-147.

© 2015 by the President and Fellows of Harvard College