Department of Pediatrics
Division of Nephrology, Enders Bldg., Rm. 561.1
300 Longwood Avenue
Boston, MA 02115 - 5737
Lab Members: 6 postdoctoral fellows
Our research focuses on how stem cell and progenitor populations in developing organs are regulated by signaling networks. Important areas of study include how transcription factors and chromatin modification proteins are involved in organ development. We have taken a genome-wide approach to identifying targets of the Wilms’ tumor-1 transcription factor (WT1) using chromatin immunoprecipitation, using chromatin from embryonic kidneys. These studies have provided evidence that WT1 regulates chromatin modification in development, and future studies will examine histone modifications in development and disease models that are regulated by WT1.
We are also studying the role of microRNAs in kidney progenitor cells, and we have begun to identify microRNAs required to maintain progenitor populations and molecular pathways regulated by microRNAs.
Our studies on organ development are being extended to several disease models. Polycystic Kidney Disease (PKD) is one of the most common genetic diseases. We are investigating roles for integrins and receptor tyrosine kinases in the pathogenesis of PKD. These studies have identified a role for Wnt signaling in PKD that we are continuing to investigate. We are also using high throughput sequencing to characterize changes in global microRNA expression in PKD, that will elucidate new roles for microRNAs in human disease.
Pandey, P., Qin, S., Ho, J. Zhou, J. and Kreidberg, J.A. Systems biology approach to identify transcriptional reprogramming and microRNA targets during progression of Polycystic Kidney Disease. (In Press, BMC System Biology 2011 Apr 25;5:56. PMID: 21518438
Qin, S., Taglienti, M., Nauli, S.M., Contrino, L.,Takakura, A., Zhou, J. and Kreidberg, J.A. Failure to ubiquitinate c-Met leads to hyperactivation of mTOR signaling in a mouse model of autosomal dominant polycystic kidney disease J. Clinical Investigation 2010 120(10):3617-3628
Hartwig, S., Ho, J., Pandey, P., MacIsaac, K., Taglienti, M., Xiang, M., Alterovitz, G., Ramoni, M., Fraenkel. E., and Kreidberg, J.A. . Genomic Characterization of Wilms’ Tumor Suppressor-1 Targets in Nephron Progenitor Cells during Kidney Development. Development. 2010 137:1189-1203
For a complete listing of publications click here.
Last Update: 8/1/2012