BBS Faculty Member - Daniel Kahne

Daniel Kahne

Department of Biological Chemistry and Molecular Pharmacology, HMS
Department of Chemistry and Chemical Biology, FAS

Harvard University
Dept. of Chemistry & Chemical Biology
12 Oxford St.
Cambridge, MA 02138
Tel: 617-496-0208
Fax: 617-496-0215
Email: kahne@chemistry.harvard.edu
Lab Members: 4 postdoctoral fellows, 16 graduate students, 2 undergraduate students
Visit my lab page here.



For many years our research group has been interested in the molecular mechanisms of various antibiotics and the fundamental cellular processes they inhibit. We have primarily focused on drugs that target bacterial cell wall biosynthesis, including the beta-lactams, vancomycin, and moenomycin. We use these molecules to study the protein machines that synthesize and degrade the bacterial cell wall. Mechanistic studies of these classes of enzymes provide insight into the factors that determine bacterial cell shape, growth, and division. Recently, we have also become interested in understanding how the structure of cellular membranes is established and maintained. This is a stereochemical problem since biological membranes are asymmetric and require proper spatial organization of their constituent lipids and proteins in order to function correctly. We use E. coli as our model system and have identified two protein complexes that are involved in assembling glycolipids and proteins in the outer membrane of Gram-negative bacteria. Our challenge now is to determine how these machines function at a detailed chemical level and to explore their potential as antibacterial targets. A combination of organic synthesis, bacterial genetics, biochemistry, and structural biology are required in these efforts.



Last Update: 6/18/2014



Publications

For a complete listing of publications click here.

 


 

Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport. D. Sherman, M.B. Lazarus, L. Murphy, C. Liu, S. Walker, N. Ruiz, D. Kahne. Proc Natl Acad Sci U S A 2014; 111:4982-7.

Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in
E. Coli. S. Okuda, E. Freinkman, D. Kahne. Science 2012; 338:1214-7.

Disulfide rearrangement triggered by translocon assembly controls lipopolysaccharide export. S.S. Chng, M. Xue, R.A. Garner, H. Kadokura, D. Boyd, J. Beckwith, D. Kahne.
Science 2012; 337:1665-8.

Reconstitution of outer membrane protein assembly from purified components. C.L. Hagan, S. Kim, D. Kahne.
Science 2010; 328:890-2.



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