Department of Stem Cell and Regenerative Biology
Howard Hughes Medical Institute
Harvard Stem Cell Institute
Richard B. Simches Research Building, CPZN 4242
185 Cambridge Street
Boston, MA 02114
Visit my lab page here.
Our lab tries to understand the molecular mechanisms underlying pluripotency and nuclear reprogramming. Pluripotency denotes the ability of cells, such as embryonic stem (ES) cells, to give rise to all cell types of the mammalian body, while nuclear reprogramming is the dedifferentiation of a specialized cell back into a pluripotent state. Reprogramming does not normally occur in vivo but can be achieved experimentally by nuclear transfer, ES cell-somatic cell fusion and by directly inducing embryonic genes in somatic cells, generating so-called induced pluripotent (iPS) stem cells.
We are studying these processes functionally by establishing transgenic and knock-out mice and by manipulating murine and human ES cells. Combined with genome-wide approaches including RNAi and chemical screening we aim to dissect the mechanisms of pluripotency and epigenetic reprogramming. Ultimately, we hope that our research will aid in attempts to generate custom-tailored cells for treating and understanding disease.
Effie Apostolou, PhD, Postdoctoral Fellow
Sihem Cheloufi, PhD, Postdoctoral Associate
Benjamin Schwarz, MD/PhD, Postdoctoral Fellow
Ryan Michael Walsh, PhD Student (BBS)
Abby Sarkar, PhD Student, (BBS)
Jiho Choi, PhD Student, (BBS)
Marti Borkent, Visiting PhD Student (Erasmus University, Rotterdam)
Nade Abazova, Visiting Student
Jennifer Cloutier, Undergraduate student (Harvard)
Mary Ann Yram, Technician
Jenna L. Norton, Administrative Assistant
Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Induced pluripotent stem cells generated without viral integration. Science 2008 Nov 7;322(5903):945-9.
Eminli S, Foudi A, Stadtfeld M, Maherali N, Ahfeldt T, Mostoslavsky G, Hock H, Hochedlinger K. Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells. Nat Genet. 2009 Sep;41(9):968-76. Epub 2009 Aug 9.
Utikal J, Polo JM, Stadtfeld M, Maherali N, Kulalert W, Walsh RM, Khalil A, Rheinwald JG, Hochedlinger K. Immortalization eliminates a roadblock during cellular reprogramming into iPS cells. Nature. 2009 Aug 27;460(7259):1145-8.
Stadtfeld M, Apostolou E, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature. 2010 Apr 25. [Epub ahead of print]
Induced pluripotency: history, mechanisms, and applications. Stadtfeld M, Hochedlinger K. Genes Dev. 2010 Oct 15;24(20):2239-63. Review.
Chromatin connections to pluripotency and cellular reprogramming. Orkin SH, Hochedlinger K. Cell. 2011 Jun 10;145(6):835-50.
For a complete listing of publications click here.
Last Update: 7/26/2012