BBS Faculty Member - David Golan

David Golan

Department of Biological Chemistry and Molecular Pharmacology

Harvard Medical School
Seeley G. Mudd Building, Room 304C
250 Longwood Avenue
Boston, MA 02115
Tel: 617-432-2256
Fax: 617-432-3833
Email: dgolan@hms.harvard.edu
Lab Members: 4 postdoctoral fellows, 2 graduate students



Our goals are to understand the molecular interactions controlling protein and lipid mobility and distribution in cell membranes, the roles these mechanisms play in interactions between cells, and the relationships between derangements in these mechanisms and the pathophysiology of disease. We have designed and constructed several time-resolved scanning laser microscopes for interactive monitoring, tracking, and manipulating of biological samples at the single-cell and single-molecule levels on the µs-ms time scale and nm distance scale. Using these instruments, we are investigating: 1) Molecular interactions in erythroid cell membranes. We aim to define the modes of motion and strengths of interactions involving individual molecules in the mature red cell membrane, and to investigate the molecular biophysics of integrin-mediated adhesive interactions in terminal erythroid differentiation. 2) Lymphocyte-erythrocyte adhesion in sickle cell disease. We aim to define the molecular mechanisms mediating adhesion of sickle red cells to activated T lymphocytes, and to investigate correlations between the level of adhesion and the pathophysiology of painful crisis episodes in patients with sickle cell disease. 3) Molecular interactions in T-cell adhesion. We aim to define the modes of motion, cell surface distribution, and two-dimensional binding interactions of T-cell adhesion molecules in natural and artificial membrane systems. 4) Quantitative analysis of the cystic fibrosis transmembrane conductance regulator (CFTR)-mediated internalization of Pseudomonas aeruginosa by lung epithelium in cell culture systems and animal models. We aim to quantify the physical properties of CFTR and related proteins at sites of contact between P. aeruginosa and lung epithelial cells, and to characterize the molecular mechanisms mediating internalization of bacteria by these cells. 5) Cellular imaging of protein-protein interactions: visualizing the dynamic regulation of endothelial nitric oxide synthase (eNOS) and caveolin in calcium-dependent signal transduction. We aim to visualize the dynamic regulation of eNOS, caveolin, and related signaling molecules in vascular endothelial cells in culture and in the intact vasculature. Graduate student rotation projects are available in each of these areas.



Last Update: 8/22/2013



Publications

For a complete listing of publications click here.

 


 

Cairo CW, Das R, Albohy A, Baca QJ, Pradhan D, Morrow JS, Coombs D, Golan DE. Dynamic regulation of CD45 lateral mobility by the spectrin-ankyrin cytoskeleton of T cells. J Biol Chem. 2010; 285:11392-11401. Epub 2010 Feb 17.

Bajmoczi M, Gadjeva M, Alper SL, Pier G, Golan DE. Cystic fibrosis transmembrane conductance regulator and caveolin-1 regulate epithelial cell internalization of Pseudomonas aeruginosa. Am J Physiol Cell Physiol. 2009; 297:C263-277. Epub 2009 Apr 22.

Karnchanaphanurach P, Mirchev R, Ghiran I, Asara JM, Papahadjopoulos-Sternberg B, Nicholson-Weller A, Golan DE. C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex. J Clin Invest. 2009; 119:788-801. Epub 2009 Mar 2.

Leader B, Baca QJ, Golan DE. Protein therapeutics: a summary and pharmacological classification. Nat Rev Drug Discov. 2008; 7:21-39. Review.



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